mApple Fluorescent Protein Antikörper

Produktnummer ABIN7273083

Der Ziege Polyklonal Anti- Antikörper wurde für IEM, IF, IHC (fro), IHC (p) und WB validiert. Er ist geeignet, in Proben von Discosoma zu detektieren.

Kurzübersicht für mApple Fluorescent Protein Antikörper (ABIN7273083)

Target: mApple Fluorescent Protein

Reaktivität: Discosoma

Wirt: Ziege

Klonalität: Polyklonal

Applikation: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Produktdetails

Kreuzreaktivität (Details): Reacts against Red Fluorescent Protein

Aufreinigung: Epitope affinity purified

Immunogen: Purified recombinant fluorescent protein produced in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Kommentare:

In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Antigendetails

Target: mApple Fluorescent Protein

Hintergrund: MApple (Apple fluorescent protein) is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mApple is a ~27 kDa protein that is optimally excited at a 568 nm and has a maximum of emission at 592 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.